Выпуск 20. 2018 (С. 23

**Размножение папоротников посредством спор в культуре in vitro (обзор литературы)**

Л.А. Шелихан, Э.В. Некрасов

Амурский филиал Ботанического сада-института Дальневосточного отделения Российской академии наук, г. Благовещенск

DOI: 10.17581/bbgi2003

Интерес к папоротникам как объектам биотехнологии определяется особенностями их развития и репродукции, знание которых важно для понимания эволюции растений. Они являются ценными декоративными и медицинскими растениями, продуцентами биологически активных веществ. Воспроизводство редких и исчезающих видов папоротников важно для сохранения видового разнообразия растений. К настоящему времени накоплен обширный материал по культивированию папоротников в условиях in vitro. Разработанные методы открыли новые возможности для углубленного изучения, практического использования и сохранения видов этой группы растений. Обзор рассматривает вопросы размножения папоротников методами in vitro при использовании спор в качестве эксплантов. Протоколы, описанные в литературе, позволяют разработать оптимальные условия для введения в культуру in vitro и размножения новых видов.

Ключевые слова: Polypodiophyta, папоротники, споры, гаметофит, спорофит, культура in vitro

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**Fern propagation via spore culture in vitro (a review)**

L.A. Shelikhan, E.V. Nekrasov

Amur Branch of Botanical Garden-Institute, Far East Branch of the Russian Academy of Sciences, Blagoveshchensk

DOI: 10.17581/bbgi2003

Full text

Ferns are highly interesting objects for biotechnology due to their special development and reproduction, knowledge of which is important for understanding plant evolution. Ferns are also valuable ornamental and medicinal plants, and they have a high potential for the production of bioactive compounds. Some fern species are rare or endangered, and their conservation is necessary for plant biodiversity. There are sufficient experimental data on the in vitro cultivation of ferns, which can significantly contribute to the development of the above‑mentioned subjects. This review considers various topics on fern propagation in vitro using spores as explants. The variety of protocols described in the literature for in vitro cultivation is useful for the multiplication of new fern species.

Keywords: Polypodiophyta, ferns, spores, gametophyte, sporophyte, in vitro culture

References

Arnautova E.M. 2008. Gametofity ravnosporovykh paporotnikov [Gametophytes of homosporous ferns]. Saint-Petersburg. 456 pp. (In Russ.)